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C other features – Bio-Rad Quantity One 1-D Analysis Software User Manual

Page 73

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Quantity One User Guide

3-22

Gamma Slider

Some images may be more effectively visualized if their data are mapped to the
computer screen in a nonlinear fashion. Adjust the Gamma slider handle to expand or
compress the contrast range at the dark or light end of the range. This is reflected in
the Transform Plot and Preview Window.

3.8.c

Other Features

Full Scale and Low-High

The Full Scale and Low-High option buttons adjust how the range of data in the
image is displayed in the Frequency Distribution histogram and Transform Plot. They
do not change how the data is displayed in the image window.

Select Full Scale to adjust the displays so that they show the full intensity range of the
image.

Select Low-High to magnify the range between the Low and High sliders. This
makes it easier to view the data if it does not occupy the full intensity range of the
image.

Log

The Log checkbox changes the way the data is displayed in the histogram so you can
better discern subtle changes in signal intensity.

Image Max/Min and Units

Image Max and Min display the range of intensity in the gel image.

The image units are determined by the type of scanner used to create the image. For
images measured in O.D.s, you can display the maximum and minimum O.D values
in the image by selecting the Calibrated Quantity checkbox. If this box is
unselected, the maximum and minimum numeric pixel values are displayed.