Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 247)

Quantity One User Guide
A-6
Note: If you know the approximate exposure time you want (
±
3 seconds), you can skip
this step and go directly to Manual Expose.
Click on the Auto Expose button to cancel Live/Focus mode and begin an automatic
exposure. The Auto Expose button will appear selected throughout the exposure.
During the auto exposure, the image is continuously integrated on the camera CCD
until it reaches a certain percentage of saturated pixels. This percentage is set in the
Options dialog box. (Default = 0.15 percent. See section A.9, Options.)
Fig. A-4. Auto Expose.
Once an image has reached the specified percent of saturated pixels, it is captured and
displayed in the video display window, Auto Expose is automatically deactivated, the
exposure time appears active in the Exposure Time field, and Manual Expose is
activated.
Note: If you are having difficulty auto-exposing your sample, you can use Manual
Expose to adjust your exposure time directly. Most applications only require an
exposure time of a few seconds, which can be quickly adjusted using Manual
Expose.
Manual Expose
If you know the approximate exposure time you want, you can click on the Manual
Expose button. Manual Expose is automatically activated after Auto Expose has
deactivated.
Click on Auto Expose;
button appears depressed
Can see Exposure Time
automatically changing