Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 254)

Appendix A. Gel Doc
A-13
as white in the image, while the maximum slider defines the pixel value that will
appear as black. The slider scale is 0–255, with the defaults set to 60 minimum and
130 maximum.
Imaging Area
These fields are used to specify the size of your imaging area in centimeters, which in
turns determines the size of the pixels in your image (i.e., resolution). When you
adjust one imaging area dimension, the other dimension will change to maintain the
aspect ratio of the camera lens.
Note: Your imaging area settings must be correct if you want to do 1:1 printing. These
are also important if you are comparing the size of objects (e.g., using the
Volume Tools) between images.
Auto Exposure Threshold
When you click on Auto Expose, the exposure time is determined by the percentage
of saturated pixels you want in your image. This field allows you to specify that
percentage.
Typically, you will want less than 1 percent of the pixels in your image saturated.
Consequently, the default value for this field is 0.15 percent.
Reminder
When this checkbox is selected, the software will warn you to turn off your
transilluminator light when you exit the Gel Doc acquisition window or when your
system is “idle” for more than 5 minutes.
Video Printing Footer Information
The checkboxes in this group allow you to specify the information that will appear at
the bottom of your video printer printouts.
Save Options
To automatically create a backup copy of any scan you create, select the Make
Backup Copy checkbox.