Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 120)

Chapter 5. Bands
5-3
When a band is quantitated, the average intensity of each horizontal row of pixels
within the brackets is calculated. Next, the number of pixel rows between the top and
bottom brackets is determined. Taken together, these result in an intensity profile for
the band.
Finally, the area under the profile curve to the baseline is integrated, resulting in units
of intensity x millimeters. This is the “trace quantity” of the band.
Note: Because the profile of an ideal band conforms to the shape of a Gaussian curve,
band profiles can be “fitted” to a Gaussian model. The band quantity can then be
quantitated from the area under the Gaussian curve. This is the best to resolve
overlapping or closely spaced bands in images. See section 5.7, Gauss-Modeling
Bands, for details.
5.2 Band Detection
The Detect Bands command automatically detects the bands in defined lanes, based
on parameters that you select.
Note: Before detecting bands, you should subtract background from the lanes using the
Lane Background command (see section 4.2, Lane-Based Background
Subtraction).
Select Detect Bands from the Band menu or toolbar. The Detect Bands dialog will
open.