Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 126)

Chapter 5. Bands
5-9
Size Scale
The Size Scale field helps distinguish between trends in signal intensity and random
intensity fluctuations. It is the number of pixels in a vertical column that are taken
together to determine whether a band is present.
The Size Scale parameter is similar to the Noise Filter in that it uses the size of
objects in the image to determine the nature of those objects. The default Size Scale
setting of 5 pixels is optimal for most gel images. It can be set to any whole number
greater than or equal to 3.
If a gel image has high levels of background noise, a larger Size Scale is appropriate.
At low noise levels, a smaller value is preferable. You can also increase the Size Scale
if the gel only has a small number of thick bands scanned at high resolution.
5.2.b Parameter Sets
You can save detection parameters for use on similar images. Enter a name for the set
in the Parameter Set Name field, and click on the Save button.
Fig. 5-5. Parameter set controls in the Detect Bands dialog.
To load a saved set of parameters, click on the Load button and select the set from the
list. To remove a set of parameters, first load it, then click on Delete.
5.2.c Default Parameters
To return all detection parameters to their default values, click on the Reset button at
the top of the form.