Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 219)

Quantity One User Guide
10-10
Fig. 10-9. Comparison Options dialog.
In the Comparison Options dialog, select All Bands to include every band in the
sample similarity comparison, or selected Classified Bands to include only matched
bands.
Next to Weighted, select Yes to use both band position and intensity when comparing
sample similarity. Select No to use only band position when comparing samples. The
next section describes how weighting is used in the similarity comparion equation.
Comparison Method
The method for computing similarity in Quantity One is the Dice Coefficient. The
formula for the Dice Coefficient is:
where S and T are vectors representing two lanes in the same band set that are being
compared.
To compute similarity, a vector is constructed that represents the bands identified in
the lane. The vector depends on the comparison options (see above) selected. If the
sim 200
Min s
i
t
i
( , )
i 1 =
B
∑
s
i
t
i
+
( )
i 1 =
B
∑
--------------------------------- ×
=
dist 100 sim – =