Bio-Rad Prestained SDS-PAGE Standards - Manual

Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547
4006024 Rev D
Bio-Rad’s prestained standards are available
in high, low, and broad molecular weight
ranges. Blue dye has been covalently attached to
the standard proteins and will not be dissociated
by normal staining or destaining. The protein
mixtures are provided in a stable aqueous solu-
tion. No reconstitution or further dilution is
required before use.
Applications
Prestained SDS-PAGE Standards provide a
quick and easy way to assess the quality of an
electrophoretic transfer and act as a control in
repetitive blotting experiments. The visibility of
the standards makes it possible to monitor the
separation of proteins while electrophoresis is
in progress, even after the dye front has run off
the gel. The standards can also be used to locate
a protein for excision from an unstained prepar-
ative gel.
Specifications
Contents Approximately 625 µg total protein*
in 33% (v/v) glycerol, 3% SDS, 10
mM Tris pH 7.0, 10 mM DTT, 2 mM
EDTA, 0.01% NaN 3 .
Storage -20 °C
Shelf life 1 year at -20 °C
Volume 500 µl
Applications 25-100
per vial
*Total protein concentration is an approximation based
on the average concentrations of multiple lots. Actual
concentrations will vary.
Coomassie is a trademark of ICI.
2 1
Reference
1. Laemmli, U. K., Nature, 227, 680 (1970).
Ordering Information
Catalog
Number
Product Description
Prestained Standards
161-0305 Prestained SDS-PAGE Standards, low range, 500 µl
161-0309 Prestained SDS-PAGE Standards, high range, 500 µl
161-0318 Prestained SDS-PAGE Standards, broad range, 500 µl
161-0324 Kaleidoscope Prestained Standards, 500 µl
161-0325 Kaleidoscope Polypeptide Standards, 500 µl
Molecular Weight Standards
161-0304 SDS-PAGE Standards, low range, 200 µl
161-0303 SDS-PAGE Standards, high range, 200 µl
161-0317 SDS-PAGE Standards, broad range, 200 µl
161-0314 Silver Stain SDS-PAGE Standards, low range, 200 µl
161-0315 Silver Stain SDS-PAGE Standards, high range, 200 µl
161-0306 Biotinylated SDS-PAGE Standards, low range, 250 µl
161-0311 Biotinylated SDS-PAGE Standards, high range, 250 µl
161-0319 Biotinylated SDS-PAGE Standards, broad range, 250 µl
161-0326 Polypeptide SDS-PAGE Standards , 200 µl
Specialty Standards
161-0310 IEF Standards, pI range 4.45-9.6, 250 µl
161-0320 2-D SDS-PAGE Standards, 500 µl
Prestained
SDS-PAGE Standards,
Low Range
Catalog Number
161-0305
Product shipped at room temperature.
Store at -20 °C upon arrival
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