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Bio-Rad Unstained SDS-PAGE Standards User Manual

Silver stain sds-page standards, low range, Reference, Ordering information

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SDS-PAGE Molecular Weight

Standards,Low Range

Specifications

Low Range

Range

14,400 to 97,400 daltons

Contents

Rabbit muscle phosphorylase b

Bovine serum albumin

Hen egg white ovalbumin

Bovine carbonic anhydrase

Soybean trypsin inhibitor

Hen egg white lysozyme

Volume

200 µl concentrated

solution

Storage

-20 °C

Shelf Life

1 year at -20 °C

Applications

400 full size gels

per vial

800 mini gels

Recommended

12.5%*

gel percentages

1

*Note:

The lowest recommended gel percentage for the low

range standards is 10%. At gel percentages of 10%

or less, one or more of the standards may migrate at

the dye front, depending on running conditions and

other factors. As a result, only four or five bands

may be visible after staining. If this should occur, an

increase in gel percentage is recommended.

Silver Stain SDS-PAGE Standards contain approximately

700 µg total protein in 50% glycerol (w/v), 300 mM NaN

3

,

20 mM Tris, and 4 mM EDTA. The proteins have been

blended to give bands of equal intensity on SDS polyacry-

lamide gel systems run according to Laemmli

1

and stained

with Bio-Rad Silver Stain or Silver Stain Plus. Different

results may be obtained when alternative silver staining

chemistries are used.

Reference

1.Laemmli, U. K.,

Nature, 227,

680 (1970).

2.Hames, B. D. and Rickwood, D.,

Gel Electrophoresis of Pro-

teins: A Practical Approach, Second Edition, p. 17, Oxford

University Press, New York (1990).

2

Ordering Information

Catalog
Number

Product Description

Molecular Weight Standards

161-0303

SDS-PAGE Standards,

High, 200 µl

161-0304

SDS-PAGE Standards,

Low, 200 µl

161-0317

SDS-PAGE Standards,

Broad, 200 µl

161-0314

Silver Stain SDS-PAGE Standards,

Low, 200 µl

161-0315

Silver Stain SDS-PAGE Standards,

High, 200 µl

161-0306

Biotinylated SDS-PAGE Standards,

Low, 250 µl

161-0311

Biotinylated SDS-PAGE Standards,

High, 250 µl

161-0319

Biotinylated SDS-PAGE Standards,

Broad, 250 µl

161-0320

2-D SDS-PAGE Standards

161-0326

Polypeptide SDS-PAGE Standards,

200 µl

Prestained Standards

161-0305

Prestained SDS-PAGE Standards,

Low, 500 µl

161-0309

Prestained SDS-PAGE Standards,

High, 500 µl

161-0318

Prestained SDS-PAGE Standards,

Broad, 500 µl

161-0324

Kaleidoscope Prestained Standards,

500 µl

161-0325

Kaleidoscope Polypeptide Standards,

500 µl

IEF Standards

161-0310

IEF Standards,

pI range 4.45-9.6, 250 µl

Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules CA 94547

4006047 Rev C

Silver Stain

SDS-PAGE Standards,

Low Range

Catalog Number

161-0314

Product shipped on dry ice.

Store at -20 °C upon arrival.

4006047C 8/28/98 03:48 PM Page 1