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F.5 calibration – Bio-Rad Quantity One 1-D Analysis Software User Manual

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Appendix F. GS-800

F-9

Fig. F-8. Entering a custom resolution (with Oversample selected).

Image File Size

The size of the scan file for the selected resolution is listed next to Image File Size. If
you do not have enough computer memory for the specified file size, an error
message will appear when you attempt to scan. If this happens, select a lower
resolution or decrease the size of the area to be scanned. (Macintosh users can also
increase the application memory partition. See your Macintosh computer
documentation for guidance.)

F.5 Calibration

The GS-800 automatically calibrates the densities of scanned images to ensure
accuracy and reproducibility of results. The GS-800 has built-in step tablets for both
transmissive and reflective scanning.

The transmissive step tablet calibrates transmissive scans from 0 to 3.0 O.D.

The reflective step tablet targets reflective scans from 0 to 2.0 O.D.

The first time you use the GS-800, you must select some settings to ensure accurate
calibration. Click on the Options button in the main acquisition window. This will
open the Densitometer Options dialog box.