Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 196)

Chapter 8. Colony Counting
8-5
Doing a Recount
To recount using different parameters, change the Sensitivity and/or Averaging
settings. This will erase the old count. Then click on Count again to recount.
Redrawing the counting region circle or clicking on the Reset button will also erase
the count.
8.4 Making and Erasing Individual Colonies
If automatic colony detection has missed or erroneously detected some colonies, you
can manually mark or unmark them directly on the image using the buttons under
Tools/Options.
Fig. 8-4. Colony counting tools.
To mark a colony, click on the Make Colony button, then click on the spot on the
image that you want to identify as a colony.
To unmark a colony, click on the Erase Colony button, then click on the colony on
the image that you want to unmark.
The colony count will change accordingly.