Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 364)

Appendix H. Fluor-S MAX
H-9
For most applications, you can select an exposure time, capture an image, study it,
then adjust the exposure time accordingly. Repeat this procedure as many times as
necessary to obtain a good image.
For chemiluminescent samples, which degrade over time and emit low levels of light,
you can select a high exposure time initially and use Live Acquire mode to save
intermediate exposures (see following section).
Preview
For shorter exposures, you can use Preview to test different exposure times. Click on
the Preview button create a preview exposure and display it in the acquisition
window.
Recommended Exposure Times and Lenses
Sample
Recommended
Exposure
Lens & Filter
1 Accessories Used
Fluorescent Stain Gel 1–20 sec. Zoom/IR None
Fluorescence End-Label Gel 10 sec.–2 min. Zoom/IR None
Fluorescent Blot 0.1–3 sec. Zoom/IR None
Chemifluorescent Blot 0.1–3 sec. Zoom/IR None
Colorimetric Gel 1–5 sec. Zoom/IR White Diffusion Plate
Colorimetric Blot 0.2–10 sec. Zoom/IR None
X-ray film 0.1–5 sec. Zoom/IR White Diffusion Plate
Weak Chemiluminescence 2 2–10 min. 50 mm Chemi Tray
(if sample is small)
Strong Chemiluminescence 2 5 sec.–1 min. 50 mm Chemi Tray
(if sample is small)
1 For sharper focusing, close the f-stop down 1–2 stops from full open while focusing.
2
For chemi applications, the 50mm lens is recommended. Always remove the 660 filter.