Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 164)

Chapter 6. Standards and Band Matching
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Standard Curve displays the Standards Regression Curve (see section 6.1.d,
Standards Regression Curve). Click on the button, then click on any lane in the image.
Other Band Set Functions
The units of the bands are displayed in the top half of the dialog, as is the matching
tolerance used. Tolerance is the minimum spacing between unique bands that you
specified when you created the band set.
The Normalization button allows you to pick a specific identified band that appears
in all the lanes to normalize the relative quantities of the other bands against (see
section 9.1, Differential Display for more information).
Click on the resize button in the lower right corner to reconfigure the dialog to its
smaller, palette version, which displays only the tool buttons and band type buttons.
To delete the band set, click on the Delete button.
To close the band set, click on OK.
Note that the band set is saved when you save the image.
6.2.c Tips for Gels Without Standards
If you are not using standards, we recommend that you load at least two lanes per gel
with a reference sample containing many if not most of your experimental bands of
interest.
Select the Match command from the menu or toolbar, and click on this reference
sample lane to create a new band set. Then select Matched Band Set from the Match
menu to open the Band Set dialog, and apply that band set to other reference sample
lanes using the Propagate Band Set command.
Propagate Band Set is a feature that not only simplifies identifying bands, it allows
the software to do some optimizations that will significantly speed up modeling.
Choose another reference sample lane that you want to apply the band set to. Start by
using the Match command to identify one or two bands in the lane, then click on the
Propagate Band Set button and click on the lane to assign the remaining band types
to the lane.