Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 363)

Quantity One User Guide
H-8
more accurately focus the camera. Then, after focusing, increase the f-stop to the
desired setting.
After you have positioned your sample, click on the Focus button and look at the
image in the acquisition window while aligning the two focusing arrows on the
camera lens. While focusing, the camera will limit its focus to a small portion of the
sample (this will not affect any zoom lens adjustments you may have made.)
When you are finished focusing, click on the Stop button.
H.4 Step III. Set Exposure Time
When you are ready to capture an image, you will need to select an exposure time.
“Exposure” refers to the integration of image captures on the CCD over a set period
of time. The effect is analogous to exposing photographic film to light.
The exposure time you select should be based on your application and your “best
guess” as to what exposure will give you the best image.
Note: The minimum exposure time in trans illuminated mode is 1 second. The
minimum exposure time in epi illuminated mode is 0.1 second.
You can enter an exposure time (in seconds) directly in the field, or use the Arrow
buttons to adjust the exposure time in 10 percent increments.
Fig.H-8. Selecting an exposure time.
The following table provides recommended exposure times for various applications.