Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 246)

Appendix A. Gel Doc
A-5
focusing on, you can place a ruler in the Gel Doc cabinet so that it is visible in the
image.
A.3 Step II. Select Image Mode
The Image Mode option buttons allow you to set the type and scale of your data.
UV
Select this mode for fluorescent samples. With this mode selected, the data will be
measured in linear intensity units.
White Light
Select this mode for reflective and transmissive samples. With this mode selected, the
data will be measured in uncalibrated optical density (uOD) units.
A.4 Step III. Acquire Image
For many white light applications, you can skip this step and save and print images
directly from Live/Focus mode.
For UV light or faint samples, you can take an automatic exposure based on the
number of saturated pixels in the image or you can enter a specific exposure time.
Note: “Exposure” refers to the integration of the image on the camera CCD over a
period of time. The effect is analogous to exposing photographic film to light
over a period of time.
Auto Expose
Auto Expose will take an exposure whose time length is determined by the number of
saturated pixels in the image. This is useful if you are uncertain of the optimal
exposure length.