Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 262)

Appendix B. ChemiDoc
B-7
At this point, if you are in UV or White image mode, Manual Expose will be
automatically activated. If you are in Chemi mode, the Freeze button will be
automatically activated.
Note: If you are having difficulty auto-exposing your sample, you can use Manual
Expose to adjust your exposure time directly. Most non-chemi applications only
require an exposure time of a few seconds, which can be quickly adjusted using
Manual Expose.
Manual Expose
If you know the approximate exposure time you want, you can click on the Manual
Expose button. In UV or White image mode, Manual Expose is automatically
activated after Auto Expose is complete.
Fig.B-5. Setting a manual exposure.
With Manual Expose activated, you can adjust the exposure time directly by changing
the number of seconds in the Exposure Time field. Type in a number or use the arrow
buttons next to the field.
In UV or White image mode, when the specified exposure time is reached, the last
captured image will be displayed in the ChemiDoc image window. The camera will
continue to integrate the image on the CCD, updating the display whenever the
specified number of seconds is reached.
Manual Expose active;
button appears selected
Exposure Time field active;
adjust number of seconds