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Bio-Rad Quantity One 1-D Analysis Software User Manual

Page 5

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Quantity One User Guide

iv

3.5. 3D Viewer ..................................................................................................... 3-10

3.6. Image Stack Tool .......................................................................................... 3-12

3.7. Colors ............................................................................................................ 3-14

3.8. Transform ...................................................................................................... 3-17

3.9. Resizing and Reorienting Images ................................................................. 3-23

3.10. Whole-Image Background Subtraction ....................................................... 3-29

3.11. Filtering Images .......................................................................................... 3-33

3.12. Invert Data ................................................................................................... 3-39

3.13. Text Overlays .............................................................................................. 3-39

3.14. Erasing All Analysis from an Image ........................................................... 3-42

3.15. Sort and Recalculate .................................................................................... 3-42

3.16. Automation Manager .................................................................................. 3-42

4.

Lanes .................................................................................. 4-1

4.1. Defining Lanes ................................................................................................ 4-1

4.2. Lane-Based Background Subtraction .............................................................. 4-9

4.3. Compare Lanes ............................................................................................. 4-13

4.4. Lane-based Arrays ........................................................................................ 4-16

5.

Bands .................................................................................. 5-1

5.1. How Bands Are Identified and Quantified ..................................................... 5-2

5.2. Band Detection ................................................................................................ 5-3

5.3. Identifying and Editing Individual Bands ..................................................... 5-10

5.4. Plotting Traces of Bands ............................................................................... 5-12

5.5. Band Attributes ............................................................................................. 5-13

5.6. Band Information .......................................................................................... 5-16

5.7. Gauss-Modeling Bands ................................................................................. 5-18

5.8. Irregularly Shaped Bands in Lanes ............................................................... 5-21

6.

Standards and Band Matching ......................................... 6-1

6.1. Standards ......................................................................................................... 6-1

6.2. Band Matching .............................................................................................. 6-13