Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 303)

Quantity One User Guide
D-14
D.6.b Calibration Settings
After you have entered the step tablet values, you can immediately calibrate by
clicking on the Calibrate Now button (in the Densitometer Options dialog box).
You can also specify how often you want the densitometer to automatically
recalibrate. Either click on the Calibrate Before Every Scan checkbox or enter a
Recalibration Interval (in minutes) in the appropriate field.
Note: With calibration turned on, the scanner will automatically recalibrate each time
you change your filter or your reflective/transmissive setting. (If you select a
different application with the same filter and light settings, it will not auto
recalibrate.)
Calibration Report
To print out a calibration report each time the densitometer calibrates, click on the
Calibration Report checkbox.
D.7 Other Options
Oversample
This feature allows you to scan at the maximum resolution of the GS-700 (42.3 x 42.3
microns) and then use spatial averaging to create an image with lower resolution. This
can result in better images at lower resolution—however, it takes longer to scan.
To turn on oversampling, click on the More Options button in the acquisition window
and click on the Oversample checkbox.
With oversampling on, you can specify your own resolution within the range of 43–
169 microns by entering values directly in the fields next to X resolution and Y
resolution in the main acquisition window.