Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 132)

Chapter 5. Bands
5-15
determined by the type of standards defined for the gel, the band’s position in the
lane, and any modeling performed on the gel (using band matching or multiple
lanes of standards) to compensate for gel distortion or smiling.
• Peak density—The intensity value of a band peak.
• Average density—The total intensity of the rows of pixels used to generate the
profile of a band, divided by the number of rows.
• Trace qty—The quantity of a band as measured by the area under its intensity
profile curve. Units are intensity x mm.
• Relative qty—The quantity of a particular band as measured by its intensity,
expressed as a percentage of either the total intensity of all the bands in the lane
or the total intensity of the lane (including the areas between bands). The
calculation method (% of Lane or % of Bands in Lane) is set in the Preferences
dialog (see section 2.5.e, Application).
• Gauss Peak Density—The intensity value of a band’s Gaussian peak (after
Gaussian modeling).
• Gaussian Trace Quantity—The quantity of a band as measured by the area
under its Gaussian-fitted profile.
• Contour qty—The quantity of a band that has been identified using the Contour
or Draw Band tools. It is the sum of the intensities of all the pixels within the
band boundary multiplied by the area of each pixel. Units are intensity x mm
2
.
• Contour area—The area (in mm
2
) inside the boundary of a band that has been
identified using the Contour or Draw Band tools.
• Calibrated qty—The quantity of a band as calculated from the trace quantity
and quantity standards. (Note that this is different than quantity determined using
volumes.) Units are user-defined.
• Normalized qty—The trace quantity of a particular band expressed as a
percentage of the quantity of a selected band type that is present in the same lane.
• Band type—The band type number of a band that has been matched and placed
in a band set.
• Band model—Displays the modeling lines across the gel that are generated by
band matching, standards, or both. These lines are used to compensate for gel
distortion or smiling.
• Tandem repeats—The number of repeated base-pair units in a band that has
been analyzed using the VNTR Calculations function (see section 9.2, Variable