H.3 step ii. position/focus – Bio-Rad Quantity One 1-D Analysis Software User Manual
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Appendix H. Fluor-S MAX
H-7
H.3 Step II. Position/Focus
Note:
When you click on the Position or Focus button, the light inside the Fluor-S
MAX box automatically turns off. This is because if you focus with the camera at
maximum aperture (see note below), leaving the light on would make it difficult
to view the image. To turn the light on while positioning or focusing, hold down
the
SHIFT
key when clicking on the button.
Position
The next step in acquiring an image is centering your gel or other object within the
camera frame. To do this, click on the Position button. The Fluor-S MAX will begin
capturing a “live” image and updating it every second.
With the Position button selected, look at the image in the acquisition window while
you position your object in the center of the platen. If you have a zoom lens on the
camera, you can adjust the magnification while you position.
You can select the Show Alignment Grid checkbox to facilitate positioning.
Fig.H-7. Fluor-S MAX alignment grid.
When you are finished positioning, click on the Stop button.
Focus
Note:
Before focusing, you should adjust the f-stop on the camera to the lowest setting
(i.e., the maximum aperture). This reduces the depth of field, allowing you to