Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 204)

9-1
9. Differential Display and
VNTRs
Quantity One has tools for studying gene expression using differential display
analysis and counting VNTRs or other repeated elements in gels.
These functions are located on the Analysis menu.
9.1 Differential Display
Differential display is a popular technique using mRNA and PCR amplification to
identify genes that are differentially expressed between and among cell types.
1
The
mRNA in a cell is amplified using RT-PCR and resolved on a DNA sequencing gel,
where it can be compared with mRNA from related cell types to determine
differential gene expression.
The Differential Display tool in Quantity One facilitates the side-by-side comparison
of the bands in a sequencing gel by highlighting the up or down regulation of bands
across lanes.
Note: You must match the bands in the gel image before using this function. See
Chapter 6 for more information.
9.1.a Normalization
Before using the Differential Display function, you must normalize the bands in the
gel for quantity across all the lanes. With the gel open and matched, select Normalize
from the Match menu and click on a distinct, well-resolved band that appears in every
lane of the gel. The quantity of that band in each lane will be set to 100, and the
quantities of the other bands in the lane will be normalized to that band. (If the band is
not present in a lane, the normalized quantity for that lane is zero.)
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