Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 152)

Chapter 6. Standards and Band Matching
6-9
Fig. 6-9. Applying a single standard value.
After you have applied the standard values to a lane, the bands in that lane will
change color to blue, indicating that they are now standards.
You are now ready to select the standards regression curve to use for calculating the
unknowns.
Removing Standard Values from Lanes
The Clear from Lane command removes all the standard values from the lane(s) you
select. Click on the button, then click on the lane or lanes from which you want to
delete the standard values.
Showing the Modeling Lines
Click on the Show Modeling Lines button in the Standards dialog to display lines
across the gel connecting the same standard bands in different lanes. Unknown bands
that fall along these lines have the same values as the standards.
To redisplay only the band numbers with no modeling lines, click on the Show Band
Types button.
6.1.d Standards Regression Curve
After you have applied the standard values to the image, you are ready to select the
regression model to use to calculate the values of the unknown bands. (Note: You
must apply the values to a lane before you can view and adjust the regression curve.)
Click here to apply
this standard value
to a particular band
on the image