Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 205)

Quantity One User Guide
9-2
See section 6.2.d, Normalizing for Quantity, for more information on normalization.
9.1.b Differential Display Searches
After you have normalized the gel, select Differential Display from the Analysis
menu. A dialog will open in which you can specify certain parameters.
Fig. 9-1. The Differential Display dialog box.
You can perform two types of searches on bands across a differential display gel: an
Outliers search or a Trends search. Both are determined by the Percentage and
Quantity values that you specify in the Differs By field.
Outliers
Select the Outliers button to highlight bands with a normalized quantity that differs
from the mean normalized quantity for that band type by the specified percentage
and/or the specified quantity. An outlier is defined as a band with normalized quantity
(n
q
) that satisfies one of the following criteria:
1. n
q
> ratio x mean and
n
q
> mean + quantity
2. n
q
< (1/ratio) x mean and
n
q
< mean – difference
Where: ratio = 1.0 + (percentage/100)