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Bio-Rad Quantity One 1-D Analysis Software User Manual

Page 170

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Chapter 6. Standards and Band Matching

6-27

If all of the known bands are in one lane, click on the Lane button, then click on
the lane with the known quantities. The entire lane will be highlighted.

Fig. 6-21. Buttons for selecting bands of known quantity.

In the lower part of the Quantity Standards dialog, the lane and band numbers of the
selected bands will appear in the Band column. The intensity of each band will also
be listed.

Entering the Quantities

Enter the quantity of each known band in the Quantity column. When you enter a
quantity, the value of the band in the Status column will change from Unknown to
Known.

Fig. 6-22. Entering the known quantities.

After you have entered a few quantities, the status of the remaining bands in the list
may change to O.R., meaning that the remaining bands are out of the current range of
values (based on their intensities and what you have already entered).