Bayer HealthCare Rapidlab 800 - Manual (Page 535)

Performance Characteristics EĆ9
Reference Methods
The following reference methods were used for the 800 systems.
Analyte Reference Method
pH IFCC reference method , * also referenced by NCCLS document C–27A. 1
pCO
2
and
pO
2
Tonometered whole blood as described in NCCLS document C21–A.
[
Gases used are traceable to NIST Certified Reference Material SRM
series 1701.
Na + and
K +
Method described in NCCLS document C29 12 , which serves as the basis
for the NIST Certified Reference Material SRM 956 using flame
photometry.
Cl – Coulometric reference method. This method, which is embodied in the
Bayer Diagnostics 925, is also used to assign values to NIST Certified
Reference Material SRM 956.
Ca ++ Internal method used.
Glucose The hexokinase/glucose-6-phosphate dehydrogenase method described
in NCCLS document RS1–A.
]
Lactate LD Manual Assay
tHb Cyanmethemoglobin Reference Method per National Committee for
Clinical Laboratory Standards (NCCLS), approved reference procedure
k
using Cary 4 Spectrophotometer.
FO
2
Hb Tonometry, where whole blood samples are tonometered with
95% O
2
, 5% CO
2
.
FHHb Tonometry, where whole blood samples are tonometered with
95% N
2
, and 5% CO
2
.
FCOHb Gas Chromatography
w
for COHb
≤
15% and Ishizawa # method using a
Cary 4 Spectrophotometer for COHb
≤
15%
FMetHb Modified Evelyn–Malloy 37 method utilizing a Cary 4 Spectrophotometer.
* International Federation of Clinical Chemistry. Reference method (1986) for pH measurement in blood.
IFCC 1987/3.
[
National Committee for Clinical Laboratory Standards. Performance characteristics for devices measuring
PO
2
and PCO
2
in blood samples; Approved Standard; NCCLS Document C21–A; (Vol 12, No. 3);
March 1992.
]
National Committee for Clinical Laboratory Standards. Glucose; Approved Summary of Methods and
Materials; NRSCL Document RS1–A; 1998.
w
Vreman, H.J., Kwong, L.K., and Stevenson, D.K., Carbon Monoxide in Blood: an Improved Microliter
Blood-Sample Collection System, with Rapid Analysis by Gas Chromatography, Clin. Chem.30, (1382 -
1386) 1984.
k
National Committee for Clinical Laboratory Standards. Reference and Selected Procedures for the
Quantitative Determination of Hemoglobin in Blood – Second Edition; Approved Standard;
NCCLS Document H15–A; (Vol 14, No. 6); May 1994.
# Ishizawa F. A Study on the Spectrophotometric Determination of Carboxyhemoglobin in Blood —
Isobestic Point Method. Jpn J Legal Med 1981, 35(3), 191–200.