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4 liquid handling (lqh), 1 liquid handling parameters – Metrohm 916 Ti-Touch User Manual

Page 396

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28.11 Dosing and Liquid Handling

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916 Ti-Touch

28.11.4 Liquid Handling (LQH)

The command LQH can be used to carry out Liquid Handling tasks with a
dosing device of the type Dosino 800. The manifold options of the Dos-
ino can be exploited in full in combination with a USB Sample Processor.
The four ports of the dosing units can be used at will as output or input
ports. This means that not only simple dosing and filling procedures are
possible. Complex Liquid Handling tasks such as pipetting or sample trans-
fers can be carried out without difficulty. Several LQH and automation
commands are required for this purpose which are best combined into
subsequences.

28.11.4.1

Liquid Handling parameters

The parameters for the Liquid Handling function are defined under [Liq-
uid Handling param.]
.

Function

Type of Liquid Handling function.

Selection

Dose | Fill | Aspirate | Eject | Exchange posi-
tion
| Change port | Compensate | End volume

Dose
The given volume is dosed. The dosing cylinder neither is automatically
filled before nor afterwards.
Fill
The dosing cylinder is filled. The valve disc then remains at the selected
port.
Aspirate
Liquid is aspirated. The dosing cylinder neither is automatically filled
before nor afterwards. The aspirating volume has to be aspirated with
a single piston stroke.
Eject
The whole cylinder content is ejected. The piston is, in contrast to the
function End volume, lowered to the stop point, i.e. past the maxi-
mum volume mark. This function should only be carried out for prepar-
ing a dosing unit before the pipetting procedure.
Exchange position
The dosing cylinder is filled. This means that, for example, air can be
aspirated via Port 4. The valve disc is then rotated to Port 2 and the
dosing drive can be removed from the dosing unit.
Change port
The stopcock is moved to the given port, but no piston movement
takes place.