Luminex 100 IS Version 2.2 User Manual
Page 82
Luminex 100 IS User Manual Version 2.2
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MAP Technology
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PN 89-00002-00-069 Rev. A
2. Click OK to continue. The command list on the Run Batch tab
indicates that the system is running. The Device Activity field on
the Status Bar indicates that the system is priming. Upon
completion, the command list indicates whether the command
succeeded with green text or the command failed with red text.
Backflush
Command
Backflush the system to perform these tasks:
•
to remove obstructions from the cuvette
•
if fluid does not flow through the waste tubing during prime
cycles or during sample acquisition
•
if fluid drips from the Sample Probe during priming and forms
puddles of fluid on the plate
During a backflush, the system draws sheath fluid from the sheath
fluid container. You do not need to supply solution in a plate. A
backflush takes about seven seconds.
To backflush obstructions from the cuvette:
1. Click Backflush on the Maintenance tab. A confirmation dialog
box appears verifying that you want to backflush the system.
2. Click OK to continue. The command list on the Run Batch tab
indicates that the system is running. The Device Activity field on
the Status Bar indicates that the system is backflushing. Upon
completion, the command list indicates whether the command
succeeded with green text or the command failed with red text.
Alcohol Flush
Command
Alcohol flush the system to remove air bubbles from the sample
tubing and the cuvette using 70% isopropanol. The cuvette is the
principal fluid pathway within the optics component of the system
where the system reads the sample. The alcohol flush takes about six
minutes.
To remove air bubbles from the sample tubing and cuvette:
1. Click Alcohol Flush on the Maintenance tab or Home tab. A
confirmation dialog box appears prompting you to place alcohol
flush solution in the reservoir.
2. Click Eject/Retract. The plate holder ejects.
3. Place the solution in the reservoir and click Eject/Retract to
retract the plate holder.
Note:
You need to remove air
bubbles from the system
because air bubbles can
interrupt the sample flow and
can distort your bead results.