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Analyze batches and multi-batches – Luminex 100 IS Version 2.2 User Manual

Page 140

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Luminex 100 IS User Manual Version 2.2

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MAP Technology

6 - 86

PN 89-00002-00-069 Rev. A

Eject/Retract

The Eject/Retract command ejects or retracts the microtiter plate
from its current position.

‹

To eject or retract the microtiter plate:

Click Eject/Retract on the Run Batch tab or the Acquisition
Detail tab. The command toggles between Eject and Retract
depending on the current status. That is, if the plate is retracted,
click Eject/Retract to eject the plate. If the plate is ejected, click
Eject/Retract to retract the plate.

Analyze Batches

and Multi-Batches

You can analyze an acquired batch using the analysis features of
Qualitative and Quantitative algorithms. The algorithm is determined
by the kit manufacturer during template creation.

Qualitative analysis—determines the results as either positive or
negative, reactive or non-reactive, and so on. The system is also
flexible in defining custom result ranges, such as negative, low
positive, high positive, and so on. Refer to the Creating Templates
section of the Luminex 100 IS Developer Guide to xMAP Technology
Version 2.2
for additional information. All determinations are based
on a single standard.

Quantitative analysis—determines the sample concentrations from
standard curves using regression methods, such as 4P or 5P logistic
curve fitting.

You may direct the system to acquire samples in replicate regardless
of batch type. For qualitative batches, replicate values are averaged
and the reported interpretation determined from this replicate
average.

Replicates in quantitative batches are based on a standard curve that
is generated by either the “Fit of all standards” or “Mean of
replicates”. The chosen curve fit is defined by the assay developer
when defining an assay template. The default is “Fit of all
standards”. Unknown samples are calculated from the standard
curve. Replicate samples are averaged to determine the reported
quantitative result denoted as “AVG”.

The system can analyze only batches that it acquires using qualitative
or quantitative templates. It does not analyze acquisitions using Data
Collection Only or Maintenance templates.

Note:

Luminex does not

recommend performing Data
Analysis while the Luminex 100
IS 2.2 system is performing data
acquisition on another batch.