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Background samples – Luminex 100 IS Version 2.2 User Manual

Page 127

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MAP Technology

Using Luminex 100 IS 2.2 Software

PN 89-00002-00-069 Rev. A

6 - 73

plate image or Command List areas on the Run Batch Tab the
Command Information dialog box does not appear.

Paste Batch

Information to a

Document

‹

To paste information from the current batch into a document:

1. Open the document where you want to paste the information.

Click the cursor at the insertion point in the document.

2. Press the Ctrl+V keys on the keyboard or right-click in the

document and select Paste from the drop-down command list.
The copied information appears at the insertion point in the
document.

Clear a Batch

From the System

The Clear Batch command clears the entire batch from the Run
Batch tab or the Message Log on the Diagnostic tab.

‹

To clear a batch from the system:

1. Click over the area to clear.

2. Press the Ctrl+X keys on the keyboard or right-click and select

Clear from the dialog box.

3. At the Clear Batch or Clear Message Log warning dialog box,

click Yes to clear and No to abort.

Background Samples

Background samples are commonly used in the assays process in the
Luminex 100 IS 2.2 system. Although recommended, the discretion
on whether to use or not is left to the assay or kit developer. If the
background samples are to be included they have been defined in the
assay template.

Background samples are samples with no active test substance. The
system uses background samples to remove assay background noise
from the sample results.

Typical background samples contain coupled beads,

assay buffer,

detection reagents, and reporter fluorophore. Background samples do
not contain target analytes.

If the background sample is designated accordingly, the system

subtracts the sample’s reported fluorescence from all samples in a
batch to report net fluorescence intensity (MFI).

If a batch contains more than one background sample, the
flourescence values of the two background samples are averaged

Note:

Once you choose to

clear the batch and verify that
you want to continue with the
command, you can regain the
cleared batch if it has not been
run by clicking Open Batch.