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Luminex 100 IS Version 2.2 User Manual

Page 147

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MAP Technology

Using Luminex 100 IS 2.2 Software

PN 89-00002-00-069 Rev. A

6 - 93

Recalculation

When you make a change, such as invalidating a standard or editing
lot information, you must recalculate the affected test. The system
provides two options, Automatic recalculate and manual recalculate.
The Auto checkbox (under Recalc button) at the bottom of the
Standards tab determines the option.

Figure 83. Recalc Button

Auto selection—In the Auto selection mode changes

automatically trigger the system to reanalyze the affected test.
This is the default mode unless you uncheck the Auto
checkbox. Uncheck Auto to use the Recalc button in the
Manual recalculate mode.

Manual recalculate—After making changes that require a

recalculation, the system enables the Recalc button. In this
mode you press the Recalc button to initiate the recalculation.
Examples of Recalc scenarios include: change formula,
change lot, validate/invalidate a standard/control, in-line lot
changes (changing Expected Conc from the Standards grid).

Invalidate or

Validate Standards

and Controls

You can invalidate or validate a standard or control in either of two
ways. Use the associated button on the bottom of the Analysis
window or use the shortcut by right-clicking in the desired Expected
Conc field.

Invalidating standards—You can invalidate or remove a standard if
doing so improves the curve fit. Invalidating standards greatly affects
the curve fit and subsequently the sample results. However, observe
caution when doing so. For further instruction on assay standard
curves and the appropriateness of invalidating or removing
standards, contact the assay kit manufacturer.

Invalidating controls—You can invalidate or remove a control in
data analysis. However, Luminex does not recommend invalidating
controls. For further instruction on assay controls and guidelines
regarding accepting or rejecting control values, contact the assay kit
manufacturer.

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To validate or invalidate a standard or control entry:

Shortcut procedure:

Note:

The Recalc button is

greyed out unless you make
changes that require
recalculation.