Luminex 100 IS Version 2.2 - Manual (Page 107)

x MAP Technology Using Luminex 100 IS 2.2 Software
PN 89-00002-00-069 Rev. A 6 - 53
• Test a batch using different parameters, such as setting a
different number of events to be collected or using a different
bead map or new formula for analysis, also using a different
template.
If you reprocess a batch with the same template parameters, the
system should obtain results similar to the original batch. If you
reprocess a batch using changed parameters, the system may obtain
different results.
When you replay a batch it labels unknown samples as Pa1, Pa2,
Pa3, and so on.
If you replay a batch containing replicates, replicate averaging will
not be calculated in data analysis.
A number of variables can affect the final test results. You may also
change the standards or controls processed with the batch or multi-
batch. These variables may effect your test results:
• minimum number of events for acquisition
• formula used to analyze the MFI values
• standards or controls validation or invalidation
• type of analysis (qualitative, quantitative, acquisition only, or
maintenance)
Reprocess
Samples Using
Replay Batch
You reprocess samples using Replay Batch from the Acquisition
Detail tab.
‹ To reprocess samples using Replay Batch:
1. Click the Acquisition Detail tab.
2. Click Replay Batch on the Acquisition Detail tab. A Browse for
Folder dialog box appears displaying the My Batches directory
(folder).
3. Select the desired batch under the My Batches folder and click
OK.
4. The Open Template dialog box appears. Select the desired
template and click Select.
5. The Run Batch tab becomes the active tab. You can monitor the
commands as they process. Switch to the Acquisition Detail tab
and monitor the data, histogram, and dot plot.
Note: The number of beads
for collection must be less
than or equal to the number
previously collected in the
original sample.