Luminex 100 IS Version 2.2 - Manual (Page 137)

x MAP Technology Using Luminex 100 IS 2.2 Software
PN 89-00002-00-069 Rev. A 6 - 83
• Fit of All Standards (default)
• Mean of Replicates
The assay developer defines the curve fit generation method while
creating the assay template.
The system default is to generate the curve fit according to the fit of
all standards method. When generating a curve fit according to the fit
of all standards, the system plots all standards to define the curve
.
If the curve is generated from the mean of replicates, the system
determines the fluorescent value for each of the replicate standards,
then calculates the mean flourescence of all replicates. The system
plots the average (mean) of the replicate standards on the standard
curve. Luminex offers this method to reduce the time it takes to
calculate the standard curve.
If the system detects that you validate or invalidate a sample, the
change effects the final results. The system flags the change and
enables the Recalculate button so you can recalculate your results
after changing data. Click Recalc to recalculate data. Checking the
auto box below Recalc causes the system to automatically recalculate
the results after each change.
Regardless of how the system generates the curve fit, the system
averages replicate test results for all samples (standards, controls, or
unknowns) to determine the “reported” test results labeled as
“AVG”.
Plate Commands
The system acquires samples based on various plate commands. The
plate commands are:
• Start Plate
• Pause
• Resume
• Cancel Command
• Cancel All
• Eject/Retract
• You can perform plate commands during sample acquisition.
• You can perform nonplate commands, that is, Prime, Reservoir
Wash, Sanitize, and so on between samples.