Luminex 100 IS Version 2.2 - Manual (Page 199)

x MAP Technology Troubleshooting
PN 89-00002-00-069 Rev. A 8 - 9
Sample Timed Out • Verify that you properly mixed the samples before dispensing
them into the wells.
• Verify that you loaded the sample to the correct well on the
plate.
• Verify that you added the correct sample volume to the well.
• Remove and sonicate or flush the Luminex XYP instrument
Sample Probe to remove possible clogs.
• Verify that the Sample Probe is properly aligned.
• Complete the following analyzer functions from the Maintenance
Tab: Backflush, Drain, Alcohol Flush and Water Wash.
• Verify that the delta cal temperature is ± 3.0 degrees. Recalibrate
if outside these limits and retry.
• Run the system controls.
• If the system controls pass, contact the kit manufacturer.
Sample Empty
Detected
This error message occurs when the system completely acquires the
entire sample volume.
• Verify that you properly mixed the samples before dispensing
into the wells.
• Verify that you loaded the sample to the correct well on the
plate.
• Verify that the sample volume added to the well is correct.
• Remove and sonicate or flush the Luminex XYP instrument
Sample Probe to remove possible clogs.
• Verify that the Sample Probe is properly aligned.
• Complete the following analyzer functions from the Maintenance
tab: Backflush, Drain, Alcohol Flush and Water Wash.
• Verify that the delta cal temperature is
±
3.0 degrees. Recalibrate
if outside these limits and retry.
• Run the system controls.
• If the system controls pass, contact the kit manufacturer.
Unknown Formula
Failure
This error message encompasses a range of mathematical errors
often indicating that the sample result was negative or invalid. This is
according to the parameters defined by the formula used to analyze
the sample results. Standards, assay controls, and unknowns may all
be flagged with the Unknown Formula Failure. A standard curve is
plotted using the MFI and expected concentration value. An
Unknown Formula Failure may occur when the system is unable to
calculate a standard concentration from the standard curve.