Luminex xMAP AbC - Manual

xM
89-30
This Q
intend
Reag
1
Reag
2
MAP
®
A
0000-00-361
Quick Guide is a
ded to replace the
gent Prep:
1.  A
gent Calcul
2.  C
a. Beads
Desired
Concen
b. Activat
i. 40
ii. 48
c. Sulfo-N
i. 50
ii. 10
d. EDC fo
i. 50
ii. 10
e. Antibo
De
De
St
f. Activat
Total r
i. 10
ii. 50
Volume
Volume
bC Kit
Rev. A
checklist of all of
e kit user manua
Allow kit to equ
lations:
Calculate the v
for coupling rea
d number of Bea
ntration of stock
Volume of
Volume of
tion Buffer for A
00 μL for reaction
80 μL for reaction
NHS for Activatio
0 μL for reactions
0 μL for reactions
or Activation step
0 μL for reactions
0 μL for reactions
ody for coupling s
esired number o
esired Antibody
tock Antibody co
Volume of A
Volume of A
tion Buffer for c
reaction volume
000 μL for reactio
00 μL for reaction
e of Activation Bu
e of Activation Bu
Quick
f the steps for pe
al (89-00002-00-3
uilibrate to roo
volumes requ
ction:
ads to couple:
vial:
f stock neede
f stock neede
Activation step:
ns of more than 5
ns of 5x10
6
Bead
on step:
s of more than 5x
s of 5x10
6
Beads
p:
s of more than 5x
s of 5x10
6
Beads
step:
f Beads to coup
y concentration:
oncentration:

Ab needed =

Ab needed =
coupling step:
e :
ons of more than
ns of 5x10
6
Bead
uffer needed = to
uffer needed =
Guide
erforming one co
319). Please refe
om temperatu
ired for each


# of be
ed =
conc o


____
ed =
______
5x10 6
Beads
ds or less
x10 6
Beads
s or less
x10 6
Beads
s or less
le:

# of beads to
St


6 ____ 10 be
μg
____

µL
n 5x10 6
Beads
ds or less
otal reaction vo
upling reaction u
fer to the user ma
ure for 20 to 3
reagent:
x10 6
x10 6
B

ads to couple
of stock vial


___beads
beads
__
mL
x10 6
µg/1x
µg/mL

couple Desire
tock Ab conc


6
___ μ
eads
1 10 b
1 mL
mL 1000 μL

 

 

 
lume – volume o
µL -
using the xMAP A
anual for comple
30 minutes
Beads /mL

e
x10 6
Beads
L

ed Ab conc
μg
beads






of Antibody
µL
Antibody Couplin
ete instructions, w
Vol
the
Volu
need
Volu
activ
Volu
activ
Volu
coup
Volu
need
ng kit (40-50016)
warnings, and pre
lume of Bead st
e coupling react
ume of Activatio
ded for activatio
ume of Sulfo-NH
vation (Step 11)
ume of EDC nee
vation (Step 14)
ume of Antibody
pling (Step 21):
ume of Activatio
ded for coupling
_
Page 1 of 2
) and is not
recautions.
tock needed for
tion (Step 4):
m
on Buffer
on (Step 8):
µ
HS needed for
):
µ
eded for
):
µ
y needed for
µ
on Buffer
g (Step 20):
____________u
2
r
L
L
L
L
L
L