Luminex 100 IS Version 2.2 - Manual (Page 103)

x MAP Technology Using Luminex 100 IS 2.2 Software
PN 89-00002-00-069 Rev. A 6 - 49
1. Gate Boundaries
2. Aggregate Beads
3. Numerical Gate Position
Figure 49. Set DD Gate Example
Histogram Buttons Four buttons and the Show Bead entry field appear at the top of the
histogram:
Show Bead Select an entry from the drop-down list to set the histogram to show
events for only one bead set [bead set number], events
within the gate, or events inside and outside the gate. Select
(default) before setting the gate.
Auto Scale Select to automatically adjust the maximum number of events shown
on the Y axis. Click during acquisition to readjust the Y axis scale.
Zoom Select to zoom or enlarge a specific area on the histogram display.
Click and drag right to left to adjust the graph’s range.
Log/Linear Select to toggle the X axis scale between logarithmic and linear
modes.
Maximize/
Minimize
Select to toggle between maximum and minimum histogram views.
•
Click Maximize (up arrow) to enlarge the entire histogram and
observe in greater details.
• Click Minimize (down arrow) to reduce the histogram down to
original size.
1
2
3
Note: The gate in effect when the
system collects data determines
which values to use to obtain the
result. Applying a gate or
changing a gate for existing data
does not change your calculated
values. The gate positions used
while collecting data are the
numerical values selected in the
template or the New Advanced
Batch (also located in the lower
corner of the histogram).
Note: The calculated data uses
only events to
determine the final result.