Luminex 100 IS Version 2.2 - Manual (Page 138)

Luminex 100 IS User Manual Version 2.2 x MAP Technology
6 - 84 PN 89-00002-00-069 Rev. A
Start Plate The Start Plate command begins the acquisition process. During
acquisition, the system draws sample from the microtiter plate for
processing.
As the system processes data, the system analyzes whether the
sample passes predetermined criteria or not. If a sample fails, the
system flags that sample and acquisition continues on to the next
sample.
‹ To start acquiring sample from a microtiter plate:
1. Check the Sample Probe vertical height adjustment and adjust if
needed. Refer to Appendix A for Sample Probe adjustment
procedure.
2. Create or open the batch that you want to process. Refer to
“Create a New Batch” on page 6-55 or “Open a Batch” on
page 6-60 for directions.
3. Prepare the microtiter plate with samples for processing.
4. Click Eject/Retract to place the microtiter plate on the plate
holder.
5. Load the microtiter plate on the plate holder with well A1 in the
upper-left corner. Ensure that you carefully follow the kit
manufacturer’s directions.
6. Click Start Plate from the Run Batch tab. The system retracts
the plate and begins processing the batch’s template commands.
It acquires the samples from the wells as the commands appear
on the template.
The system takes the specified volume for testing. A horizontal
progression bar appears on the Run Batch tab to show the status
of each sample acquisition. After each sample is analyzed,
approximately 160 µL of sheath fluid is dispensed into each well.
As the system analyzes each well, the well shows either a check
mark in a green well for successful acquisitions or an X mark in a red
well for failed acquisitions. Samples may fail for many reasons,
including lack of sufficient events, Cancel command, heater out of
range, or plate cancellation.
If Auto-Start Acquisition is selected, the system analyzes samples
according to the kit manufacturer’s template commands.