Luminex 100 IS Version 2.2 - Manual (Page 152)

Luminex 100 IS User Manual Version 2.2 x MAP Technology
6 - 98 PN 89-00002-00-069 Rev. A
Replicate Averaging
The data analysis function supports replicate sampling. The function
calculates each sample as an individual sample, then averaged to
obtain a replicate average.
• Standards tab—displays standards and controls average values.
• Samples tab—displays samples average values.
Replicate averages are displayed with AVG in the Loc (location)
column.
The AVG entry appears immediately after the wells being averaged
or at the end of the list depending on what you select under Sort
(Sequentially or Alphabetically). See “Sample Progress Sorting” in
the following section.
If the system fails to determine the results for a replicate due to an
excessive skew of one of the samples, you can invalidate the out of
tolerance value. Use the Invalidate Standard (F4) or Invalidate
Control (F6) buttons at the bottom of the Analysis window.
However, this only fixes standards/control, not necessarily patient
sample. If any of the replicate standards and/or controls are
invalidated the “Avg” results reflects the remaining “good” ones.
The system flags the failed sample so you may calculate the replicate
set’s average without including the failed result in the equation.
Sample Progress Sorting
You can sort the batch samples on the Standards Tab and Sample
Tab. See Figure 89.
Figure 89. Analysis Window—Batch Sort Feature
You will find this feature helpful in viewing batches containing
replicate samples. Replicate samples are defined as samples with
identical sample identifications. Replicate standards, controls, and
unknown samples are not always acquired in sequential wells and
thus make sample viewing difficult. When viewing samples
alphabetically all replicate samples are displayed together with the
replicate sample’s average (AVG), then individual samples. If you
view replicate batches sequentially, each sample is displayed in the
order it was acquired, followed by a replicate average summary
section.
Note: You predefine standard
and control replicates in the
template. You define unknown
sample replicates in batch setup
indicated by replicate Sample ID.