Luminex 100 IS Version 2.2 - Manual (Page 102)

Luminex 100 IS User Manual Version 2.2 x MAP Technology
6 - 48 PN 89-00002-00-069 Rev. A
statistics to export through the Data Export tab on the Options
dialog box (select Tools Menu, then Options).
• %CV—Coefficient of Variation = Standard Deviation/Mean x
100%
• Count—Gated events, if gates are set
• Peak—Histogram bar with the highest value
• Std. Deviation—Standard deviation from the mean. SD
Formula:
• Median—The middle value in a list of ascending ordered
values
• Mean—Average of all values in the list
• Trimmed—All trimmed statistics remove the lower and upper
five percent of the extreme statistic values, then used for the
%CV, Count, Mean, Peak, or StdDev.
Histogram and Gates
This section provides an overview of the Histogram and Gates.
Details are provided in the Luminex 100 IS Developer Guide for
xMAP Technology Version 2.2.
The histogram appears in the lower-left section of the Acquisition
Detail tab. See Figure 49. The histogram defaults to display the
Doublet Discriminator (DD) on the X axis and Events on the Y axis.
Doublets appear when two microspheres stick together, creating
undesired results. When you enable the gate, two vertical red, dashed
lines appear to represent gate positions determined by the template or
settings established within the New Advanced Batch. After setting
the gate, everything outside the gate is ignored.
s
Σ x x – ( )
2
n 1 – ( )
---------------------- =
Note: You cannot change the
gate position during batch
acquisition. You can change the
gate positions before data
acquisition, after the system
finishes acquiring data, or after
pausing the system. However, the
change is visual. The data is still
collected according to the gate
positions set in the assay template
or New Advanced Batch set-up.