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Glossary – Luminex 100 IS Version 2.2 User Manual

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PN 89-00002-00-069 Rev. A

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Glossary

ambient temperature

The temperature of the surrounding environment.

analyte

A substance that is detected through assay analysis. Each test or bead
set will test for a specific analyte.

analyzer

This term is used to refer to the Luminex 100 analyzer.

APD

Avalanche Photo Diode; Measures the excitation emission intensity
of the color coding classification dye mixtures inside the
microsphere and the amount of light scattered as particles pass by the
lasers.

background (noise)

That portion of a bead set result that can be attributed to excess
reporter molecules in the solution, nonspecific binding, or
fluorescent spillover from another fluorochrome.

batch

A group of samples that will be processed using a selected template.

bead

Shorthand terminology for an xMAP microsphere.

calibration

A process used to normalize the settings for the reporter channel,
both classification channels, and the doublet discriminator channel
for the Luminex 100 IS. Calibration ensures optimal and consistent
microsphere classifications and reporter readings.

calibrators

xMAP microspheres used to normalize the settings for the reporter
channel, both classification channels, and the doublet discriminator
channel for the Luminex 100 IS.

CL1

Refers to dyes embedded in the microsphere. Also see classification
channel.

CL2

Refers to dyes embedded in the microsphere. Also see classification
channel.

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