8 separating columns and suppressor module, General information on separating columns, Selection of the sample loop – Metrohm 732 IC Detector User Manual
Page 39: General information on suppressor module, One-channel system without suppressor module, Two-channel system without suppressor module, One-channel system with suppressor module, Leak testing and conditioning
2 Installation
732 IC Detector / 733 IC Separation Center
32
2.8
Separating columns and suppressor module
2.8.1
General information on separating columns
New IC separating columns are normally filled with solution and
sealed at both ends. Before the column is installed in the system, it
must be ensured that this solution is freely miscible with the eluent
used (check manufacturer's specifications).
The IC separating columns and precolumns currently available from
Metrohm are listed in section 7.3.2. A test chromatogram and an infor-
mation leaflet is provided with each column. You will find additional in-
formation concerning these columns in the 8.732.2003 Metrohm Mono-
graph «Ion chromatography» and in special "Application Bulletins",
which are available on request free of charge from your local Metrohm
agency.
When you install the column, always ensure that this is inserted cor-
rectly in accordance with the flow direction shown (arrow must point
upwards).
2.8.2
Selection of the sample loop
Selection of the sample loop depends on the separating column used.
Normally, the following sample loops are used:
Anion columns
100
µ
L
Cation columns
10
µ
L
Columns for suppressor technique
20
µ
L
Depending on the instrument version, the following sample loops are
installed in the 733 IC Separation Center:
Version
Valve
Sample loop
Volume
2.733.0010
A
6.2620.120 (steel)
100
µ
L
2.733.0020
A
6.2620.120 (steel)
100
µ
L
B
6.2620.100 (steel)
10
µ
L
2.733.0030
A
6.1825.210 (PEEK)
20
µ
L
2.733.0120
A
6.1825.220 (PEEK)
100
µ
L
B
6.1825.230 (PEEK)
10
µ
L
2.733.0130
A
6.1825.210 (PEEK)
20
µ
L
If desired, the built-in sample loop can be replaced by one of the sam-
ple loops available as an option (see section 7.3.1).