Metrohm 732 IC Detector - Manual (Page 35)

2 Installation
732 IC Detector / 733 IC Separation Center
28
2.7 Precolumns
2.7.1 General information on precolumns
The use of easily exchangeable precolumns protects the separating
columns and prolongs their lifetime. The precolumns available from
Metrohm (see section 7.3.2) are either real precolumns or precolumn
cartridges, which are used together with the 6.2821.050 Twin cartridge
holder or a 6.2821.040 Cartridge head.
New IC precolumns are normally filled with solution and sealed at
both ends. Before the precolumn is installed in the system, it must be
ensured that this solution is freely miscible with the eluent used
(check manufacturer's specifications).
2.7.2 Precolumns with twin cartridge holder
The precolumn cartridges are installed in the 6.2821.050 Twin cartridge
holder as follows (see Fig. 15):
1 Install cartridge
• Insert inlet capillary 75 75 with end piece for precolumn cartridge
in Manufit housing 62 62.
• Insert outlet capillary 72 72 with end piece for precolumn car-
tridge in Manufit pressure screw 57 57.
• Remove end caps from the precolumn cartridge 74 74 (the steel
mesh 73 73 and gaskets 59 59 are already inserted in the car-
tridge).
• Fit the two capillary end pieces onto the precolumn cartridge
74 74 (comply with the flow direction if specified on the column).
• Screw Manufit pressure screw 57 57 and Manufit housing 62 62
firmly together.
2 Connect precolumn
• Fit connectors to inlet capillary 75 75 of the assembled precol-
umn (see section 2.5).
• Connect inlet capillary 75 75 either using 6.2620.060 Coupling to
the column connection capillary 67 67 mounted on the injection
valve (see section 2.6.4) or directly to connection "4“ of injec-
tion valve A or B.
• Shorten outlet capillary 72 72 of the precolumn to ca. 5 cm and
mount the connectors (see section 2.5).
3 Rinse the precolumn
• Place a beaker beneath outlet capillary 72 72.
• Switch on 709 IC Pump, rinse precolumn for ca. 10 min with
eluent and then switch off 709 IC Pump.