Eppendorf epBlue with MultiCon User Manual

Page 58

epBlue Application Editor - Creating and editing applications
epBlue™ with MultiCon
English (EN)

6.3.3.1 Example of a dilution series

This example explains the concept of a dilution series. The example does not describe the full application.

Objective

• 24 samples are in a rack with 24 positions and should be diluted 1:1000.
• The dilution should be completed in 3 program steps with dilutions of 1:10.
• First, a single-channel dispensing tool is used. An eight-channel dispensing tool will then be used to

reach a higher speed.

Procedure

• 200

µL of each of the samples will be transferred to the 96-well plate using the

Sample transfer

command. The dilutions are carried out in this plate.

• 225

µL of the diluent will be transferred from the 300

mL reservoir to each of the empty wells of the

96-well plate using the

Reagent transfer

command.

• 25

µL of the sample will be aspirated from column 1 using the

Dilute

command and will be mixed with

respectively 225

µL of the diluent in the columns

2, 3 and 4. The mixing process will be repeated twice

in the following columns.

In this example, each dilution step is a 1:10 dilution. The required 1:1000 dilution will be achieved with the
third 1:10 dilution. The volume aspirated from the undiluted sample also applies for the dilution steps.

Creating dilution series

Transferring undiluted samples to the destination labware

1. Pattern of the

Sample transfer

command:

The source position and destination position are the same color. epBlue detects the pattern and
suggests the next field.