Eppendorf epBlue with MultiCon - Manual (Page 55)

epBlue Application Editor - Creating and editing applications
epBlue™ with MultiCon
English (EN)
55
Enter the dispensing speed of the liquid in the Dispense speed field. Input range: 0.2 mm/s – 110 mm/s.
Select a low dispensing speed if you dispense larger volumes in empty vessels. A lower dispensing
speed decreases the risk of liquid spraying back. Use larger vessels to achieve better mixing results due
to a higher speed.
Enter the blow-out delay in the Blow delay field. Input range: 0 ms – 99999 ms.
The delay is the break between the end of the dispensing during liquid dispensing and blow-out. This
setting has an effect on the
Transfer type Pipette .
If you use liquids with high wetting behavior (e.g., solutions which contain proteins or detergents,
organic solvents) and delayed drain-off characteristics (highly viscous liquids), delay the blow-out. The
residual liquid collects in the lower part of the tip prior to the blow-out.
If you use liquids with low wetting behavior (e.g., water, saline solutions), set the blow-out delay to
0 .
Enter the dispensing speed of the blow-out in the Blow speed field. Application.: 0.2 mm/s – 110 mm/s.
Using liquids that tend to form bubbles on the outlet of the pipette tip increases the dispensing speed of
the blow-out. Decrease the dispensing speed if liquid sprays back into the vessel. This setting has an
effect on the
Transfer type Pipette .
In the Blow movement field, enter the path of the piston during blow-out. Input range: 0 % – 100 % as
percentaged value of the setting Initial stroke .
No blow-out will be carried out during when 0 is entered. The full piston path is part of the dispensing
step.
When entering
100 , the entire piston path is available for the blow-out. The blow-out will be conducted
using the Blow delay and Blow speed parameters.
Increase and decrease the Blow movement along with the Blow speed setting. This setting only has an
effect on the Transfer type Pipette .
In the Initial stroke field, enter the path the piston takes before the liquid aspiration. Input range: 0 % –
100 % of the available path, dependent on the type of dispensing tool.
The higher the value, the larger the amount of piston path that is available for the blow-out after liquid
dispensing. To decrease the amount of residual liquid in the pipette tip after dispensing, increase the
value. To decrease the formation of foam during dispensing, decrease the value. Reducing the value can
increase the risk of dispensing errors. A blow-out cannot be conducted if
0 is entered.
If you change the value, the pipette tips will be automatically changed before the liquid aspiration due to
technical reasons.
In the Prewetting field, enter the number of piston movements for pre-wetting the pipette tips. Input
range: 0 – 9 cycles
Non-wetted pipette tips can be pre-wetted to create the same conditions for dispensings.
Wetting the pipette tips:
• For liquids with low vapor pressure.
• For liquids with reduced surface tension and delayed drain-off characteristics.