Eppendorf epBlue with MultiCon - Manual (Page 57)

epBlue Application Editor - Creating and editing applications
epBlue™ with MultiCon
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57
6.3.3 Dilute command
The Dilute command is used to create serial dilution series, e.g., for PCR standards. A defined volume is
pipetted several times from one vessel to the next.
Abb. 6-13: Principle of the Dilute command
Fig. 6-13: Principle of the Dilute command
The parameters of the command Dilute are identical to the command Sample transfer (see p. 48).
If the Dilute command is carried out within one labware the source positions and destination positions must
not overlap.
1. Fill the destination positions with a diluent. Use the Reagent transfer command.
2. Define the number of samples to be diluted using the Number of samples command.
3. In the pattern, define the number of dilution steps. The pattern is limited by a series or column.
4. Enter the options for the Dilute command.
5. To achieve a good mixture of sample and diluent, activate the Mix > Mix after dispensing option.
The sample and diluent will be mixed after each dispensing.
6. To mix the undiluted sample before the first aspiration, activate the Mix before aspirating option.
Proceed as follows to enter the pattern for the Dilute command:
1. Select the source position of the undiluted sample.
2. Select the destination positions of the dilution steps. The number of positions defines the number of
dilution steps.
3. Repeat the procedure for each source position.
Undiluted Sample 1. Dilution 2. Dilution 3. Dilution
Diluent Diluent Diluent