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Eppendorf epBlue with MultiCon User Manual

Page 50

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epBlue Application Editor - Creating and editing applications
epBlue™ with MultiCon
English (EN)

50

6.3.1.2

Sample transfer

>

Options

command

Abb. 6-9: Sample transfer > Options

Fig. 6-9:

Sample transfer

>

Options

Aspirate from bottom

Aspirate liquid from the base of the vessel. When using several vessels with
different volumes, enter the lowest filling volume of these vessels (

Sample

transfer

>

Parameter

).

Elution from filter

Aspirate liquids from the supernatant in filter plates. Available if in the

Parameter

window the

Pipette

radio button is selected.

Dispense from top

During liquid dispensing, position the pipette tips 3

mm – 4

mm below the

edge of the vessel. When using several vessels with different volumes, enter
the highest filling volume of these vessels (

Sample transfer

>

Parameter

).

Change Tips

Defined time when the pipette tips are changed.

when command finished

Eject pipette tips after the end of the command. The liquid will be aspirated
with the same tip from all source labware positions.

before asp. for next
destination well

Eject the pipette tips before the liquid from the next source position is
aspirated. Liquid from the same source position will be aspirated with the same
tip. Liquid from the next source position will be aspirated using a new tip.

before each aspiration

Eject pipette tips prior to each aspiration. Liquid will always be aspirated with
a new tip. Additional volume will be discarded in the waste container along
with the pipette tip.

keep tips, do not change
tips

Do not eject pipette tips. Liquid will be aspirated with the same tip from all
source positions. The tips are also used in the next command. If the transfer
type changes in one of the following commands, or the

Mix

command is used,

the tips will be ejected.

after XX aspirations

Pipette tips will be ejected after a defined number of aspirations.

Restore tips

Pipette tips will be placed back into the position of the box they were removed
from.
Is only executed if the tips within an application are reused for the same source
vessel.
The

restore tips

checkbox is only available with specific settings of the

Change

Tip

option.