Eppendorf epBlue with MultiCon User Manual
Page 52
epBlue Application Editor - Creating and editing applications
epBlue™ with MultiCon
English (EN)
52
6.3.1.4
Sample transfer
>
Liquid types
Liquid types are defined in epBlue.
Tab. 6-1:
Defined liquid types in epBlue
Dispense
Distance between the pipette tip and base of the vessel during dispensing. If
the dispensing position is above the vessel, the distance will be corrected to
the height of the vessel.
Information on mixing liquids can be found in the hardware operating manual.
NOTICE! Contamination of the dispensing tool due to high mixing speed.
If the mixing speed is too high, liquid may enter the dispensing tool.
If the liquid has a low viscosity or tends to foam, set a low mixing speed.
Use demineralized water to test the mixing settings.
Use pipette tips with filter.
Information on dispensing liquids can be found in the hardware operating manual.
Liquid type
Optimized for
50 µL tips:
pipetting as of
50 µL tips:
dispensing as of
Remarks
Alcohol
75%
Mixture of
75
%
ethanol
and 25
%
water
1 to 3
µL
3
µL
• Wash reagent in kits for
nucleic acid purification. See
applications.
Alcohol
98%
Alcohol 98
%
1
µL
3
µL
• New pipette tips are
pre-wetted with the liquid to
be aspirated.
Prewetting
• For multidispensing with
300
µL pipette tips, the
distance to the filter with
300
µL aspiration is very low.
To prevent the filter from
becoming wetted, pipetting
should be avoided with an
aspiration of 280
µL or more.
Glycerol
Mixture of
40
%
glycerol
and 60
%
water
1
µL
5
µL
• Liquid type for enzyme
solutions.
• If the glycerol content of the
enzyme solutions is
significantly lower, liquid type
Water
can be used.