UVP Life Science - Manual (Page 127)

Perform 1D Analysis
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Apply a Molecular Weight Standard to a Lane
• Calibrate a Lane
• Stretch Factor
• Manual Placement of Weights
• Exact Placement of Bands
Calibrate a Lane
• First identify all lanes and bands in the image.
• Select the lane to calibrate. [ Edit Lanes and Bands ]
• From the 1D Analysis Action Tab > Lane Profile Menu select Calibrate Lane.
• A new Molecular Weight window opens. On tab Step 1: Select MW standard click on the
standard to use and then select Next. If the standard is not on the list, click Add to add a
standard to the list (see Adding a Molecular Weight Standard to the Library ).