UVP Life Science - Manual (Page 144)

LS Software User Guide
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Once the data points are selected to graph, the software allows the user to select the type of curve or line
to fit to the data points.
• In the Graph tab of the Concentration window, select the curve model desired from the drop
down menu.
The software has several possibilities for curve models:
• Least square line: a straight line (polynomial degree 1);
• Least square quadratic: a binomial curve (polynomial degree 2);
• Polynomial 3rd degree: a polynomial curve of degree 3;
• Polynomial 4th degree: a polynomial curve of degree 4;
• Point-to-point: connects the points directly;
• Best Fit: Selects the curve with the highest ’r;Goodness of Fit’ value.
Note: In using polynomial curve types, make sure that there is at least one more data point selected
than the degree of the curve e.g., if a Polynomial 3rd degree is selected, there needed to be at
least four data points.
The software automatically and immediately fits the curve model chosen to the data points as the various
models are selected. In the curve model list, it shows the Best Fit for the curves as they are graphed. The
goodness of fit is found from the coefficient of determination (also known as "r-squared"). The goodness-
of-fit value ranges between 0.0 and 1.0. A value of 1.0 for the goodness of fit indicates a perfect fit.
Remove Concentration Calibration
To remove all calibration information including data points plotted on the graph and curve lines, go to 1D
Analysis Action Tab > Lane Profile and select Concentration. The Concentration Window pops up.
• Select the Data points tab in the Concentration Window then select Delete all. A window will pop
up asking the user to confirm the removal all calibration data.
• Click Yes or No. By clicking on Yes all calibration data is removed and a new analysis can be
started.
Note:
Changing the background correction method changes net intensity values and therefore
invalidates concentration calibration. If the user changes the background correction method, the
software will ask the user to confirm deletion of all concentration data. Answering Yes is the
same as selecting Delete all in the Data points tab.
Note: Moving the bands or resizing them also changes their net intensity values. As a result, the user
will see the word "Custom" appear in the data-source column (Concentration Calibration
Window), instead of the name of the specific band. When all lanes and bands information is
deleted, the user will be asked if all corresponding Concentration Curve data should be
removed.