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Luminex 100 IS User Manual, Version 2.1 User Manual

Page 84

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100 IS S Users Manual Version 2.1

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MAP Technology

7 - 24

PN 89-00002-00-061 Rev. A

Drain the System

The cuvette in the system can be drained temporarily and refilled in
preparation for running. Draining the system helps to remove debris
from the bottom of the cuvette. We do not recommend routinely
draining the cuvette. Use Drain only for troubleshooting purposes.

When draining, you do not need to supply solution. Draining takes
approximately two minutes and should be followed by an alcohol
flush with 70% isopropanol.

Any fluid that drains from the system drains to the reservoir as the
default. However, you can set the system to drain to any unused well
on the microtiter plate. The drain function normally expels 125 µL of
fluid.

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To drain the system:

1. Click Drain. A confirmation dialog box appears.

2. Click the drop-down arrow to the right of the Drain button. An

image of the microtiter plate and reservoir appears in the plate
holder.

3. Select a location in which the fluid can drain using the image of

the microtiter plate and reservoir, and click Eject/Retract. The
plate holder ejects.

4. Verify that the location you indicated is empty and click Eject/

Retract to retract the plate holder.

5. Click OK to continue. The command list on the Run Batch tab

indicates that it is draining. Upon completion, the command list
indicates whether the command succeeded with green text or the
command failed with red text.

Soak the Probe

Use the Soak command to prevent salt crystals from forming in the
probe due to exposure to air. Soaking the probe replaces sheath fluid
in the probe with water. You should perform the soak function at the
end of each day.The system uses at least 250 µL of distilled water.

Note:

Ensure that the location

you select to expel fluid to is
compatible with the volume
expelled.