Luminex 100 IS User Manual, Version 2.1 - Manual (Page 53)

PN 89-00002-00-061 Rev. A 6 - 1
System Calibration
System Calibration
and Verification
Overview
xMAP calibrator microspheres are used to normalize the settings for
the reporter channel, both classification channels, and the doublet
discriminator channel for the Luminex 100 IS.
xMAP control microspheres are used to verify the calibration and
optical integrity for the system.
You must calibrate at least once a month and:
• following installation
• if the system is powered off
• if the system is moved
• if a part is replaced
• if the delta calibration temperature shown on the system monitor
is more than ±3 degrees.
Each step usually takes less than one minute. You must run xMAP
controls after each calibration.
Once calibrated, the calibration values remain until you calibrate
again. You can track system calibration and verification results
through the Calibration Trend Report and the System Control Trend
Report.
Ensure that you warm up the system before calibrating.
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