Luminex 100 IS User Manual, Version 2.1 - Manual (Page 24)

Luminex 100 IS User Manual Version 2.1 x MAP Technology
3 - 2 PN 89-00002-00-061 Rev. A
Photodiodes and a photomultiplier tube receive fluorescent signals
from xMAP microspheres. The Luminex 100 analyzer digitizes the
waveforms and delivers the signals to a digital signal processor
(DSP). Proprietary algorithms function with the DSP to greatly
increase sensitivity.
xMAP Microspheres
The xMAP microspheres are highly uniform, polystyrene particles
that have been crosslinked during polymerization for physical and
thermal stability. Varying amounts of fluorochromes embedded
within each microsphere give each microsphere set an unique
fluorescent signal. To ensure the stability of this signal, it is essential
to protect the microspheres from light. Follow package
instructions for storage procedures for xMAP microspheres and
assay kits.
xMAP calibrator microspheres are used to normalize the settings for
the reporter channel, both classification channels and the doublet
discriminator channel for the Luminex 100 analyzer.
xMAP control microspheres are used to verify the calibration and
optical integrity of the system.
Software Overview
This section provides a brief overview for using the Luminex 100 IS
version 2.1 software.
With the Luminex 100 IS, you work with assay kits provided by a kit
manufacturer. A template is included with each kit and is imported to
the Luminex 100 IS. The template includes a sequence of commands
required for the assay.
Once you select the template, you enter sample data into a “batch.”
Sample input can be done quickly with either the keyboard or a
barcode scanner. A batch can include as many samples as you have
for the assay and can include multiple microtiter plates. You can
even group multiple batches together into a multi-batch for efficient
processing. You can process a batch immediately or choose to
archive the batch for testing later.
As testing proceeds through the plate, you will see the display update
to show which wells have been processed. Progress is shown in both
graphic and tabular form.
Note: Protect the xMAP
microspheres from light.